differentiated properties and proliferation of arterial smooth muscle cells in culture

 

 

 

 

Thyberg J. Differentiated properties and proliferation of arterial smooth muscle cells in culture. Int. Rev.Production of platelet-derived growth factor-like molecules by cultured arterial smooth muscle cells accompanies proliferation after arterial injury. Key words: Mesenchymal stem cells Systemic sclerosis Fibrosis Vascular smooth muscle cells Differentiation Transforming growth factor-. Word count: 3482 (erlaubt 3000). ABSTRACT Objectives Obliterative vasculopathy and fibrosis are hallmarks of systemic sclerosis (SSc). 2.7 Vascular Smooth Muscle Cell (vSMC) Culture 2.7.1 vSMC Phenotype vSMCs represent a dynamic cell[118] P. Neuville, Z. Yan, A. Gidlof, M.

S. Pepper, G.K. Hansson, G. Gabbiani, A. Sirsjo, Retinoic acid regulates arterial smooth muscle cell proliferation and phenotypic features in vivo Regulation of differentiated properties and proliferation of arterial smooth muscle cells.58. Desmouliere A, Rubbia-Brandt L, and Gabbiani G. Modulation of actin isoform expression in cultured arterial smooth muscle cells by heparin and culture conditions. These results suggest that hypoxia-induced proliferation is mediated by inducing E2F1 in chicken pulmonary arterial smooth muscle cells.Isolation and culture of chicken pulmonary arterial smooth muscle cells (PASMCs). 2.4. Cell Culture and Treatments. Human PASMCs were purchased from ATCC (Manassas, VA, USA).B. Deng, J.

Du, R. Hu et al MicroRNA-103/107 is involved in hypoxia-induced proliferation of pulmonary arterial smooth muscle cells by targeting HIF-1, Life Sciences, vol. 147, pp Smooth muscle cells (SMCs) were seeded on the scaffolds, and proliferation andBiopolymers are widely used to fabricate tissue engineered grafts due to their mechanical properties, biocompatibility3.2. Cell culture, proliferation and inltration in electrospun scaffolds with different compositions. Extracellular matrix components strongly influence the differentiated properties of isolated rat arterial smooth muscle cells during in vitro cultivation. The attachment and spreading of the cells on a substrate of fibronectin or a 105-kDa Smooth muscle strips cultured for 14 days also exhibited regular fluctuation of intracellular calcium, whereas cultured smooth muscle cells did not.12. Thyberg J (1996) Differentiated properties and proliferation of arterial smooth muscle cells in culture. Differentiated properties and proliferation of arterial smooth muscle cells in culture. 1998. J Cell Biol 1986.Exp Haematol 1995. Diverse effects of fibronectin and laminin on phenotypic properties of cultured arterial smooth muscle cells. and Thyberg J. Rehkter MD. . Differentiated properties and proliferation of arterial smooth muscle cells in culture.Production of platelet-derived growth factor-like molecules by cultured arterial smooth muscle cells accompanies proliferation after arterial injury. 3. Arterial smooth muscle cells were set up in primary culture in control medium (open symbols) or medium containing nicotine (closed symbols)Gown 1980), may act together with or indepen- dently of nicotine to influence differentiated properties and proliferation of arterial smooth muscle cells. Pulmonary vascular remodeling is a significant pathological feature of hypoxia-induced pulmonary hypertension (HPH), while pulmonary artery smooth muscle cell (PASMC) proliferation plays a leading role in pulmonary vascular remodeling. The phenotypic transition of Vascular Smooth Muscle Cells (VSMCs) into osteoblastic/chondrogenic-like cells is critical for the development of calcification in CKD patients. Osteocytes, terminally differentiated osteoblasts, have recently emerged as major regulators of calcification in bone. IFN-gamma is an inhibitor of proliferation for several cells, including vascular smooth muscle, and is also an inducer of differentiated properties forWe have therefore investigated whether IFN-gamma affects the expression of alpha- smooth muscle actin in cultured arterial smooth muscle cells. 3.0 characterization of isolated and cultured urethral smooth muscle cells.126. Thyberg, J Differentiated properties and proliferation of arterial smooth muscle cells in culture. Int Rev Cytol, 1996. 169: p. 183-265. Abstract. Abnormal proliferation of vascular smooth muscle cells (VSMCs) is an important feature of atherosclerosis, restenosis, and hypertension.Stimulation of human aortic SMCs in culture with mitogenic concentrations of serum, thrombin, basic fibroblast growth factor, and the lipid peroxidation We examined the effect of hypoxia on proliferation and osteopontin (OPN) expression in cultured rat aortic vascular smooth muscle (VSM) cells.Quiescent cultures of VSM cells were exposed to hypoxia (3 O2) or normoxia (18 O2) in a serum-free medium, and cell proliferation as well as the in human coronary artery smooth muscle cells and in differentiating mouse ES- cells.ES-cells show an almost unlimited proliferation capacity in cell culture and can retain their pluripotency. Mouse ES- cells are also characterized by a relatively short cell cycle time (12-15 h) with a short G1 phase. 5 - 1979, Ross R (1979) The smooth muscle cell in culture. Physiol Rev 59 1 61 . 6 - H. Koyama, E. W. Raines, K. E. Bornfeldt, J. M. Roberts, Ross, 1996aines EW, Bornfeldt KE, Roberts JM, Ross R (1996) Fibrillar collagen inhibits arterial smooth muscle proliferation through regulation of Cdk2 7. Thyberg, J.: Differentiated properties and proliferation of arte-. rial smooth muscle cells in culture.notypic properties of cultured arterial smooth muscle cells. 3.1 Cell culture. Human aortic smooth muscle cells (hASMCs) were purchased from Cascade Biologics (Invitrogen) and cultured according to manufacturer instructions using26. Thyberg, J et al Regulation of Differentiated Properties and Proliferation of Arterial Smooth-Muscle Cells. Such properties hold over many orders of arterial branching, indicating that mechanisms exist for their maintenance during development and outgrowthCell Culture and Transfection Small artery smooth muscle cells were obtained by the explant method from mesenteric small arteries of mice (WT and If placed in cell culture, most myoblasts will proliferate if enough fibroblast growth factor (FGF) orc-Met is a tyrosine kinase receptor that is required for the survival and proliferation of migratingDeleting myogenin results in nearly complete loss of differentiated muscle fibers and severe loss of 34. Travo P, Barrett G and Burnstock G. Differences in proliferation of primary cultures of vascular smooth muscle cells taken from male and female rats.Materials and methods: Cell Culture- PAC1: Established cell line (17), rat pulmonary arterial smooth muscle cells were cultured in 199 Thyberg, J. (1996) Differentiated properties and proliferation of arterial smooth muscle cells in culture, Int Rev Cytol 169, 183265.PubMedCrossRefGoogle Scholar. In fact, endothelial cells of developing arterial, venous, and lymphatic vessels express different genes andThe recruitment and proliferation of pericytes and smooth muscle cells to form a vessel wall depend onHemopoietic cells can survive, proliferate, and differentiate in culture if, and only if Differentiated autologous endothelial and smooth muscle cells, isolated from the patientsBAKOV L, WILHELM J, HERGET J, NOVOTN J: Influence of macrophages and macrophage-modified collagen I on the adhesion and proliferation of vascular smooth muscle cells in culture. Abstract. Thrombin and collagen induced the release of a growth factor from washed rabbit platelets which requires a serum factor derived from platelet poor plasma for maximum proliferation of the smooth muscle cells. Related documents. The calcium antagonist nifedipine inhibits arterial smooth muscle cell proliferation.Modulation of actin isoform expression in cultured arterial smooth muscle cells by heparin and culture conditions. Abstract. Smooth muscle cell proliferation plays an important role in neointimal thickening after vascular injury and may contribute toIn the present study, we report a novel series of compounds that inhibit smooth muscle cell proliferation and arterial intimal thickening after balloon angioplasty. G. Fager, G.K. Hansson, A.M. Gown, D.M. Larson, O. Skalli, G. Bondjers, Human arterial smooth muscle cells in culture: Inverse relationship between proliferation and expression of contractile proteins, Vitr. Brown JJ, Dusredieck G, Fraser R. Hypertension and chronic renal failure. Br Med Bull 1971 27:128-135. Burke JM, Ross R. Collagen synthesis by monkey arterial smooth muscle cells during proliferation and quiescence in culture. Smooth muscle cells were isolated from adult rat aorta by collagenase digestion, grown in primary culture in the presence of 10 whole blood serum (WBS), and studied by quantitativeThe calcium antagonist nifedipine inhibits arterial smooth muscle cell proliferation (1985) Nilsson Jan et al. A similar transition from a contractile to a synthetic phenotype occurs when smooth muscle cells are established in culture. Accordingly, an in vitro system has been used extensively to study the regulation of differentiated properties and proliferation of these cells. The use of induced pluripotent stem cells differentiated into vascular cells could be useful (145) in better stratifyingNitrite potently inhibits hypoxic and inflammatory pulmonary arterial hypertension and smooth muscle proliferation via xanthine oxidoreductase-dependent nitric oxide generation. Earlier studies with cultured pulmonary artery smooth muscle cells required either PKC Sahai A, Cole LA, Clarke DL, Tannen RL: Rocking promotes differentiated properties in LLC-PK1 Alipui C, Ramos K, Tenner TE: Alterations of rabbit aortic smooth muscle cell proliferation in diabetes mellitus. Thyberg J. Differentiated properties and proliferation of arterial smooth muscle cells in culture. Int Rev Cytol 1996169:183265. [PubMed: 8843655] 19. Chamley-Campbell J, Campbell GR, Ross R. The smooth muscle cell in culture. Reduced proliferation and delayed cell cycle progression of G-1 treated CASMCs cells.SM22 and -smooth muscle actin were increased in G-1 treated cells in a concentration and time-dependent manner.Cells were cultured in ibidi -dish culture insert until they reached confluence. Abstract. Pulmonary Arterial Hypertension (PAH) is a progressive devastating disease characterized by excessive proliferation of the Pulmonary Arterial Smooth Muscle Cells (PASMCs). Inhibition of smooth muscle cell proliferation in injured rat arteries: interaction of heparin with basic fibroblast growth factor. J Clin Invest.26. Thyberg J. Differentiated properties and proliferation of arterial smooth muscle cells in culture. Int Rev Cytol.

Objectives: After endothelial injury, smooth muscle cells (SMCs) in the arterial media are modified from a contractile to a sympathetic phenotype.A similar change occurs in culture and then in vitro system has been established as a useful model in which to study the control of SMC differentiation. Smooth muscle cell cultures were obtained from the aortas of prepubertal guinea pigs.Regulation of differentiated properties and proliferation of arterial smooth muscle cells. J Thyberg, U Hedin, M Sjlund, L Palmberg, B A Bottger. Differentiated vascular smooth muscle cells demonstrate a very low rate of proliferation, appropriate contractility to contractile cues and express SMC specific genes, such as smooth muscle alpha-actinHypoxia inhibits proliferation of fetal pulmonary arterial smooth muscle cells in vitro. Documents. [International Review of Cytology] Volume 169 || Differentiated Properties and Proliferation of Arterial Smooth Muscle Cells in Culture. To date, pericytes have demonstrated the ability to differentiate into adipo-cytes, osteoblasts, fibroblasts, smooth muscle cells, and phagocytesCell viability and proliferation assays Growth factor/cytokine assays Optimization of cell culture conditions Cell number determination. In this study we aim to evaluate the in vitro effects of ibuprofen on proliferation and migration of human coronary artery smooth muscle cells and on endothelial cells. Methods. Cell proliferation was evaluated by trypan blue exclusion. Contribution of smooth muscle cells to the arterial wall mechanics.Smooth muscle cell nuclei density and wall thickness remain constant along the culture periods.Stretch affects phenotype and proliferation of vascular smooth muscle cells. Introduction. Vascular smooth muscle cells (VSMCs) are the main component of the arterys medial layer.Normal VSMCs are not terminally differentiated cells with very low rates of proliferation and secretion (Rzucidlo et al 2007 Cecchettini et al 2011). 106. Backov L, Herget J, Wilhelm J. Influence of macrophages and macrophage-modified collagen I on the adhesion and proliferation of vascular smooth muscle cells in culture. Physiological Research / Academia Scientiarum Bohemoslovaca.

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